According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Our microplate. Use the provided 3. and luminescence microplate reader validation DATA SHEET. A. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Vrla used the device to quantify the concentration of a small molecule produced by. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. ” Promega Corp. July 17, 2023. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. The VersaMax reads 96-well microplates. Average StDev (mP) 1. Multimode Readers Comparison Chart. Flexible for 6-well to 384-well microplates and cuvettes. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. 00. A single-mode plate reader is a good starting point. robotics-automation-compatible. Read More. 1 sec. Instrument settings are listed in Table 1. 96-well: 0. Read the microplate Step 1: Place the microplate in the reader. 3. High. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The FilterMax F3 can read 96-well and 384-well plates. Paradigm: Delta mP. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. #ID3-STD) L-Tryptophan (Sigma cat. The purposes of the study were: 1) to determine the optimal wavelength settings for three cell lines. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. #3676). 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Monochromatic filter is equipped. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. It also integrates with the powerful SoftMax Pro Software for. Highly selective for dsDNA over ssDNA or RNA. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1 mL/well of media. #T-0254) Lysozyme (Sigma cat. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. 1 FP • Fluorescence Polarization detection for fluorescein- or . Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. ValitaTiter Plus 96-well (1 plate) VAL004. Read time* 96 wells < 15 sec. 96-well microplate at 200 µL/well. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. The Multiskan Sky microplate reader is an absorbance plate reader. absorbance, fluorescence, and luminescence with the added functionality of. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. No filter change with dual monochromator optics. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. #G7294). In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. , Sigma cat. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. Learn more 0200-7008 6- to 1536-well plates. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. best-in-class data . Jon Cuccui, Brendan W. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. Or scan up to 384 wells at any time to determine the best assay conditions. Unparalleled performance on a personalized platform. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. We would like to show you a description here but the site won’t allow us. emax and Vmax microplate readers. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. . Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. flat meniscus), but is approx 15 mL. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. Molecular Devices SpectraMax 384 Plus Microplate Reader. Omnia® Compatible Microplate Reader Documentation Version No. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. #W50-100). The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. #W50-100) SpectraMax® ABS Plus Microplate Reader. Microplates . Learn more about how absorbance is measured, and some key applications that utilize. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. $489. The SpectraMax ABS. The procedure enables direct translation of any published equation. 5. Timothy Bolus, MPM, MBA, Compliance Program Manager. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Plates can put in the drawer both in landscape and portrait positions. 10. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. The CellTiter-Glo assay from Promega uses luciferase enzyme. #0310-5227) 96-well cell wash head (Molecular Devices Cat. U-bottom: 30-40 µl. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. In simplest terms, a plate reader measures and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Figure 2. Determine DNA concentrations precisely and . ValitaTiter 96-well (Pack of 20). SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Equipped with scanning, shaking and kinetics modules. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Samples were run in triplicate. Paradigm: Delta mP. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Step 3: Click “Settings” and a screen will appear (Figure 1). Documentation:1. for 96- and 384-well microplates • Detect dim samples with unsurpassed sensitivity • Avoid saturation issues with nine orders of dynamic range • Enable robust performance with autorinse injectors • Validation and compliance tools SpectraMax® L Microplate . Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. #N207) LAL Reagent Water (Lonza cat. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. absorbance, fluorescence, and luminescence with the added functionality of. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Read one sample or up to 384 in a. 1700 Online: Sales Offices. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan molecular devices Japan, osaka. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Click on the crosshair and move it so that it is centered over the well image. After 5, 7, 11, and 13 days. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. Bioz Stars score: 86/100, based on 1 PubMed citations. The SpectraMax L reader is upgradeable to dual-channel configurations for a 2-fold. #12-639) White 96- and 384-well microplates (Greiner cat. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. Molecular Devices' microplate readers. It features a modular design that allows users to add or change detection cartridges according to their needs. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Fluorescence Microplate Reader Comparison Chart. This reader's dual monochromators for. Contents of wells were pipetted up and down several times to mix. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 012 fmol 0. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Open the catalog to page 5. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. #PLUS 384) Methods. 3. 0 OD Photometric resolution: 0. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. microplate . A control well was set up to verify. book Page 9 Wednesday, July 7, 2010 2:09 PM. Less background noise gives a better limit of detection and so a relative increase in usable signal. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. Microplate reader assays requiring infrared (IR) or red-shifted. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. i3x. Running both standard spectrophotometer and microplate. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Specifications for the SpectraMax L microplate reader. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. 4. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Use black microplates. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 2. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Microplate Spectrophotometers. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. GxP regulated industry assessments of microplate readers. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. SpectraMax 250 User. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. About;. 5°C at 37°C. Please refer to the Obsolete Microplate Reader Models for more information. Go to Product Details. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. 2 2600A ValitaTiter. 2 mm x 86 mm) Height:. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Softmax Pro GxP Software. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. We would like to show you a description here but the site won’t allow us. OEM Molecular Devices All Microplate Reader, Top r. microplate readers. 0200-6263. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. personalized as your research. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Top or bottom detection is available with a simple click of a button. Outer wells are aligned for proper viewing with FLIPR. 1. 984. microplates or in a cuvette at a. Molecular Devices VersaMax Microplate Reader Specifications. Insert the filled microplate into the drawer, matching well A1 with position A1. Expt#3 . We would like to show you a description here but the site won’t allow us. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are formed in single well constructs 12. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Built in near field communication (NFC). 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. NanoRacks added it to their line of commercial . in space. Fluorescence Microplate Reader Comparison Chart. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Published Dec 16, 2020 | Updated Dec 16, 2020. MycoAlert Reagent was added to each well and incubated at room temperature for 5. Learn more. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. With various on-board features and. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Comprehensive data analysis software. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. style. 1-color 96-well . Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. ValitaTiter Plus 96-well (1 plate) VAL004. As a result, assay flexibility is consequence-free. 6. Molecular Devices i3x. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. #G7294). Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. In addition, the iD5 reader measures TRF and FP and. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 0 500 1000 1500 2000 2500 3000 3500 time (sec. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplate Formats: 6, 12, 24, 48, 96, 384. Flexibility to read a variety of plate formats. Total volume (except deep. 2 OD at 200 nm. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. Infinite 200 Pro Microplate Reader. BSA concentration (µg/mL) Average OD. explore more. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. 0. Technical specifications. Both readers were heated to 37ºC prior to setting up . SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. The VersaMax and SpectraMax 190 read 96-well microplates. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. DNA concentration (ng/mL) Average RFU. 54: 1. Reconstitution of reagents. AquaMax 4000 Microplate Washer (Molecular Devices Cat. Reconstitution of. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. A microplate reader as . The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. Wavelength Range. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . New SpectraMax iD5 Multi-mode Microplate Reader. #ABS PLUS) Methods. 03 pM 384-well: 0. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. explore more. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. iD5. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. 0200-6262. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. Four wash/rinse bottles are included and configurable for both 96- and 384. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. reagent basin for ease of pipetting into. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. The EMax Plus reader measures flat and round 96-well microplates. 7. Photometric and kinetic analysis mode. 1 pM 1536-well: 0. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. for ValitaTiter Assays. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. 2 Pages. Microplate Readers. Keywords: fluorescence polarization, trf, fret, igg. When reading at wavelengths. 04 sec. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Molecular Devices has provided scientists with . Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 5°C at 37°C. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). . Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The system provides options for 20-, 40-,. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Reactions catalyzed by firefly and *Renilla luciferases. #PLUS 384) Methods. 6 L (with SpectraMax i3x System) Title: SpectraMax MiniMax 300 Imaging Cytometer | Molecular. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Materials. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. < 0. Figure 2-23: Bottle holder To install the bottle holder: 1. When reading at wavelengths. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. the assay. 100 fmol 384-well (75 µL) 0. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. g. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. 54: 1. ZERO BIAS. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. . Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 98 Bovine serum albumin mg/mL O. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. Application Area: Spectrophotometer for molecular biology. Visible absorbance -.